Methods The retrospectively observational research had been performed. From December 2018 to Summer 2020, 17 patients with deep wounds who were admitted into the Affiliated Hospital of Zunyi healthcare University came across the addition requirements and were most notable study, including 7 men and 10 females, aged 12 to 72 many years. The wound aspects of patients after debridement were 7 cm×3 cm to 11 cm×7 cm. Two longitudinal lines had been situated through the midpoint regarding the armpit, the posterior exceptional iliac spine, while the protruding point for the sacroiliac combined, and three transverse lines were situated 5, 10, and 15 cm below the midpoint regarding the armpit amongst the two longitudinal lines, in other words. two longitudes three transverses method, leading to two trapezoidal places. And then the thoracodorsal artery perforators in 2 trapezoidal places were explor flap. The technique is easy and trustworthy. The thoracodorsal artery perforator flap designed and gathered centered on this technique has good clinical results in restoring deep wound, with minimal donor web site damage.Objective To explore the epidemiological characteristics and therapy effects of clients with hydrofluoric acid burns in hands. Techniques A retrospective observational study ended up being performed. The medical documents of 229 customers with hydrofluoric acid burns in hands who have been admitted to Zhejiang Quhua Hospital from January 2008 to December 2020 and came across the addition requirements had been gathered. The following analytical data of customers had been gathered, including sex, age, variety of affiliated enterprise, hydrofluoric acid size small fraction, damage website, complete burn location, prehospital time, amount of medical center stay, length of wound healing, whether hypocalcemia and hypomagnesemia took place or not on entry, whether surgery intervention was performed or otherwise not, and whether scar sequelae occurred or perhaps not. Solitary factor and multivariate logistic regression analysis were used to monitor out of the risk elements affecting surgery intervention and scar sequelae of all the patients and customers whose hydrofluoric acid mass small fraction ended up being 9–0.68, -14.78–4.70, and 10.14-16.86, correspondingly, P less then 0.05 or P less then 0.01); multivariate linear regression analysis showed that both hydrofluoric acid size small fraction and prehospital time were the separate threat aspects affecting the length of wound recovery of clients (with β values of -5.84 and 0.09, respectively, 95% self-confidence Tau and Aβ pathologies periods of -10.59–1.08 and 0.05-0.12, respectively, P less then 0.05 or P less then 0.01). Conclusions The majority of clients with hydrofluoric acid burns in hands are young and middle-aged males. Variety of affiliated enterprise, hydrofluoric acid size small fraction and prehospital time would be the threat factors that impact the therapy results of customers with hydrofluoric acid burns off in fingers.Objective to analyze the changes of heparin-binding protein (HBP) in severe burn patients during impact stage and its effects on person umbilical vein endothelial cells (HUVECs) and neutrophils in vitro. Practices potential observational and experimental study techniques were used. Twenty serious burn clients whom met the inclusion requirements and were admitted into the division of Burns and plastic cosmetic surgery of Affiliated Suzhou Hospital of Nanjing health University from August to November 2020 were included in serious burn group (12 men and 8 females, elderly 44.5 (31.0, 58.0) years). During the same period, 20 healthier volunteers with typical physical assessment leads to the unit’s real Examination Center were recruited into healthy control group (13 guys and 7 females, elderly 39.5 (26.0, 53.0) years). Enzyme-linked immunosorbent assay (ELISA) method was utilized to identify the necessary protein genetic association appearance quantities of HBP and tissue inhibitor of metalloproteinase 1 (TIMP-1) in plasma of patients within 48 hours after injuryor P less then 0.01); compared with that in PMA alone team, the good appearance rate of CD63 protein in the neutrophils additionally the necessary protein phrase quantities of HBP and MPO into the culture supernatant of cells in rTIMP-1+PMA team were all notably decreased (with t values of 5.26, 2.83, and 1.26, correspondingly, P less then 0.05 or P less then 0.01). Conclusions The phrase standard of HBP within the plasma of serious burn clients is increased during surprise phase. HBP can cause HUVECs to secrete TIMP-1 in vitro, and TIMP-1 can reduce the appearance of CD63 molecule in peoples neutrophils.Objective To isolate and cleanse a bacteriophage against methicillin-resistant Staphylococcus aureus (MRSA), also to evaluate its genomic information and biological attributes. Methods The experimental study techniques had been used. MRSA (hereinafter referred to as number bacteria) solution had been collected through the injury of a 63-year-old female client because of the median sternum incision infection admitted to the 2nd Affiliated Hospital of Army Medical University (the next Military healthcare University). The bacteriophage, named bacteriophage SAP23 was isolated and purified from the read more sewage of this Hospital by sewage co-culture method and double-layer agar plate technique, additionally the plaque morphology ended up being observed. The morphology of bacteriophage SAP23 ended up being seen by transmission electron microscope after phosphotungstic acid negative staining. The entire genome of bacteriophage SAP23 ended up being sequenced with NovaSeq PE15 system after its DNA was prepared by sodium dodecyl sulfonate/protease cleavage plan, and genomic ana a short latent period, and certainly will lyse MRSA effortlessly.